Xl := ComObjCreate("Excel.Application")
path = C:\Users\Admin\Desktop\1\Шаблон. Центр тестирования. Сахарово.  чел..xlsx
Xl.Workbooks.Open(Path)
Xl.Visible := true

Gui,+AlwaysOnTop        
Gui, Add, CheckBox, vDDD, DDD
Gui, Add, CheckBox, vAAA, AAA
Gui, Add, CheckBox, ...
Gui, Add, CheckBox, ...

Gui, Add, Button, Default gButtonGo, Go

Gui, Show, w200 h400, ФИО
Return

ButtonGo:

Gui, Submit, NoHide
   If DDD = 1
xl.workSheets(1).Cells.(first := xl.workSheets(1).Cells.(xl.workSheets(1).Rows.Count, "O").End(xlUp := -4162).Row + 1, "O").value := "пример1"  else
    else
.....
If AAA = 1
	xl.workSheets(1).Cells.(first := xl.workSheets(1).Cells.(xl.workSheets(1).Rows.Count, "O").End(xlUp := -4162).Row + 1, "O").value := "пример2"
else
.....